Perturbation of the mucosa-associated anaerobic gut microbiota in streptozotocin-induced diabetic rats

Our aim was to map the gut region-specific differences of mucosa-associated microbiome distribution in a streptozotocin-induced diabetic rat model. Tissue samples from duodenum, ileum and colon were collected 10 weeks after onset hyperglycaemia analyse microbiota using next-generation DNA sequencing. Striking observed duodenum between control rats. A significant invasion aerobic genus Mycoplasma apparent diabetes, abundance anaerobic phylum Firmicutes decreased massively. It is noteworthy that insulin treatment eliminated apparently restored environment mucosa. In increased samples. Although proportion Proteobacteria moderately, its composition changed significantly, induced only minor alterations. colon, slightly, relative number bacteria Bacteroidetes strongly as compared values, this increase more significant. Chronic has most prominent effect on duodenum.